Due to its thick waxy coating, " M . leprae " stains with a carbol fuchsin rather than with the traditional Gram stain.
32.
The diagnosis is suspected, when Gram-negative diplococci are seen on Gram stain of a centrifuged sample of CSF; sometimes they are located inside white blood cells.
33.
The test, which can be performed in a few minutes, is known as a Gram stain, after Dr . Hans Gram, the Danish physician who developed it.
34.
Later on, Lauer, Reller and Mirret performed a similar study, compared acridine orange with the Gram stain for detecting the microorganisms in cerebrospinal fluid and other clinical materials.
35.
Although L-forms can develop from Gram-positive as well as from Gram-negative bacteria, in a Gram stain test, the L-forms always colour Gram-negative, due to the lack of a cell wall.
36.
The results showed that the acridine orange is a simple, inexpensive, rapid staining procedure that appeared to be more sensitive than the Gram stain for detecting microorganism in clinical materials.
37.
It is best treated with a macrolide like azithromycin and a third-generation cephalosporin like ceftriaxone . " H . ducreyi " gram stain appears as " school of fish ."
38.
The bacteria, classified as gram-negative because of their reaction to the Gram stain test, can cause severe pneumonia and infections of the urinary tract, bloodstream, and other parts of the body.
39.
All gram-positive bacteria are bounded by a single-unit lipid membrane, and, in general, they contain a thick layer ( 20 80 nm ) of peptidoglycan responsible for retaining the Gram stain.
40.
"The gram stain has been an important tool in analyzing bacteria for more than a century, but it's amazing to me that we're still using a procedure that's effectively out of the Stone Age,"
