Activity of 3 H-NIS on cerebral cortical homogenates in mice show a K d of 0.80 + 0.11 nM and a B max of + 12 fmol / mg protein.
32.
The technique is used to homogenize cells and tissues, release intact organelles, prepare cell membranes, release labile biochemicals, and produce uniform and repeatable homogenates without subjecting the sample to extreme chemical or physical stress.
33.
Saturation analysis is used in various types of tissues, such as fractions of partially purified plasma from tissue homogenates, cells transfected with cloned receptors, and cells that are either in culture or isolated prior to analysis.
34.
In whole midgut homogenates, alpha-glucosidase activity increases slowly up to 18 hours after the blood meal, then rises rapidly to a maximum at 30 hours after the blood meal, whereas the subsequent decline in activity is less predictable.
35.
The effects of prion source ( via infected brain homogenate and purified PrP Sc ) and dose on penetrance ( proportion of animals eventually exhibiting clinical signs of scrapie ) and incubation period ( time to onset of clinical symptoms ) was evaluated.
36.
"Dr . Arichi, the first author, has now admitted in writing that he spiked some of the ovary homogenates . . . with calculated quantities of stock virus, resulting in the titers reported in the paper, " said the retraction.
37.
It was first made in 1989 . " In vitro " binding experiments on brain homogenates showed it to have a binding affinity between that of MiPT and 5-MeO-MiPT, both of which are known to be active psychedelics in humans.
38.
In contrast to previously described methods for detection of PrP Sc in brain homogenates, these techniques, when used to study brain homogenates, do not use seeded polymerization, amplification, or enzymatic digestion ( for example, by proteinase K, or PK ).
39.
In contrast to previously described methods for detection of PrP Sc in brain homogenates, these techniques, when used to study brain homogenates, do not use seeded polymerization, amplification, or enzymatic digestion ( for example, by proteinase K, or PK ).
40.
My variable was heating the homogenate in an oven that was set to 80?C . Unfortunately, I didn't have a thermometer, so I left it in there for what seemed a good long time ( about 15 or 20 minutes ) for it to reach that temperature.
