In the replication factory model, after both DNA helicases for leading strands and lagging strands are loaded on the template DNAs, the helicases run along the DNAs into each other.
32.
Initiation of eukaryotic DNA replication is the first stage of DNA synthesis where the DNA double helix is unwound and an initial priming event by DNA polymerase ? occurs on the leading strand.
33.
While the leading strand can use a single RNA primer to extend the 5'terminus of the replicating DNA strand, multiple RNA primers are responsible for lagging strand synthesis, creating Okazaki fragments.
34.
This discontinuous synthesis results in the lagging strand being more exposed which allows for easier beta-mediated annealing of the oligo to the target DNA than when compared to annealing to the leading strand.
35.
The leading strand is primed once, and the lagging strand is primed approximately every 1000 ( + /-200 ) base pairs ( one primer for each Okazaki fragment on the lagging strand ).
36.
The leading strand is synthesized continuously and in juxtapose to the leading strand; the lagging strand is replicated through short fragments of polynucleotide ( Okazaki fragments ) in a 5'to 3'direction.
37.
The leading strand is synthesized continuously and in juxtapose to the leading strand; the lagging strand is replicated through short fragments of polynucleotide ( Okazaki fragments ) in a 5'to 3'direction.
38.
DNA Pol III uses one set of its core subunits to synthesize the leading strand continuously, while the other set of core subunits cycles from one Okazaki fragment to the next on the looped lagging strand.
39.
Once an RNA primer has been added by a primase to the 3'end of the leading strand, DNA synthesis will continue in a 3'to 5'direction with respect to the leading strand uninterrupted.
40.
Once an RNA primer has been added by a primase to the 3'end of the leading strand, DNA synthesis will continue in a 3'to 5'direction with respect to the leading strand uninterrupted.
