Additionally, protoplasts of plants expressing fluorescent proteins in certain cells may be used for Fluorescence Activated Cell Sorting ( FACS ), where only cells fluorescing a selected wavelength are retained.
32.
In protoplast culture, the plant cell can be isolated with the help of wall degrading enzymes and growth in a suitable culture medium in a controlled condition for regeneration of plantlets.
33.
If cell wall is completely lost, the bacterium is referred as a protoplast if penicillin was used on gram-positive bacteria, and spheroplast when used on gram-negative bacteria.
34.
When cells of opposite mating types are mixed in a nitrogen-deficient mating medium, mt (-) and mt ( + ) cells pair with each other and release protoplasts.
35.
Currently the fans ( and players ) maintain good relations with "'BBkitni OwiDska "', as well of course their protoplast "'Karmazyn Warszawa " '.
36.
A third way relies on protoplasts _ plant cells that have had their tough walls removed with enzymes, leaving the cell without a wall but with a plasma membrane around it, Rathore says.
37.
During concave plasmolysis, the plasma membrane and the enclosed protoplast partially shrinks from the cell wall due to half-spherical, inwarding curving pockets forming between the plasma membrane and the cell wall.
38.
The protoplast is a light blue-green color, appearing dark or brown due to optical effects of gas-filled vesicles; this can be useful as a distinguishing characteristic when using light microscopy.
39.
Single cells, plant cells without cell walls ( protoplasts ), pieces of leaves, stems or roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones.
40.
The coloration of the protoplast is a light blue-green, appearing dark or brown due to optical effects of gas-filled vesicles; this can be useful as a distinguishing characteristic when using light microscopy.
