Fertile harlequin rasbora eggs require approximately 18 hours to hatch at a temperature of 28 �C . Upon hatching, the fry are translucent, around three to four millimetres long, and remain attached to the leaf upon which the eggs were laid for a further 12 to 24 hours, during which the yolk sac is absorbed.
32.
The clade formed by these two genera was found to be sister to a clade including many danionin genera as well as some rasborin genera such as " Rasbora ", " Trigonostigma ", and " Boraras ", making the danionin group paraphyletic without these rasborin genera based on these results.
33.
Their results differed from those of Liao et al ., and their conclusion was to synonymise the four new genera plus " Boraras " and " Trigonostigma " with " Rasbora ", based on an incomplete knowledge of relationships within the group, an approach they describe as " more conservative ".
34.
Being mainly an open water fish in the wild, using plant thickets for refuge from predation when danger threatens, the eye-spot rasbora is at home in a well-lit aquarium facilitating the growth of more demanding aquarium plants, though the fish is likely to appreciate the thoughtful provision of some floating plants for additional cover.
35.
The temperature range of the harlequin rasbora is usually cited as 22 �C to 27 �C ( 72 �F to 81 �F ) ( for example, Walker, 1971, p . 101 ) but the fish is capable of living in temperatures from 21 �C to 28 �C, and indeed usually breeds at around 28 �C ( 82.4 �F ).
36.
The harlequin rasbora is a shoaling fish, and should be kept in a group comprising a minimum of six individuals, though shoals of larger numbers are preferable not only from the standpoint of the well-being of the fish, but from an aesthetic standpoint a large shoal of harlequin rasboras presents a striking vista even to non-fishkeepers, and their active disposition in the water adds to the spectacle.
37.
Bushy plants such as " Cabomba " may also be used in the aquarium, but this genus of plants requires intense lighting, while the " Cryptocoryne " and " Aponogeton " species prefer more subdued lighting, as does the harlequin rasbora, so if the aquarist wishes to have a variety of plants in the aquarium, other species that prefer subdued lighting are better choices.
38.
B . brigittae ", the only species involved in the study ) is included in clade three alongside " Horadandia atukorali ", " Rasboroides vaterifloris ", " Trigonostigma heteromorpha " and three species previously included in Rasbora but moved into new genera by the authors : " Trigonopoma gracile ", " T . pauciperforatum " and " Rasbosoma spilocerca ".
39.
Being a peaceful species, the harlequin rasbora may be maintained in a community aquarium set-up with other similarly sized and peaceful aquarium fishes, including many of the small characins from South America ( the similarity in water chemistry of the two habitats for these different fishes has already been covered ), assorted small barbs, danios, other small rasboras, " Corydoras " catfishes, " Otocinclus " catfishes and others.
40.
The specific name " heteromorpha ", translating from the Greek as " differently shaped ", alludes to the fact that the body shape of this species differs from that of other members of the genus " Rasbora ", and as a consequence of this and assorted other factors ( including the difference in breeding technique ), the fish was assigned to a new genus, " Trigonostigma ", and is thus now known to science as " Trigonostigma heteromorpha ".
