CDNAs obtained from reverse transcription of mRNA population extracted from the cells in varying physiological and experimental conditions are used as immobilized probes.
32.
The revised hypothesis was later formulated, in part, to accommodate reverse transcription ( which both Howard Temin and David Baltimore discovered in 1970 ).
33.
Using Reverse transcription polymerase chain reaction and a more rigorous nested primer system they found evidence for splice variants of the ETV6-NTRK3 fusion gene.
34.
They also concluded from quantitative reverse transcription-PCR ( qRT-PCR ) analysis that the five consecutive genes are co-regulated by the same BCG1-calcineurim transduction pathway.
35.
CaMV contains a circular double-stranded DNA molecule of about 8.0 kilobases, interrupted by nicks that result from the actions of RNAse H during reverse transcription.
36.
This is particularly important in retroviral infections since viral integration into the host genome occurs quickly after entry and reverse transcription and is largely irreversible.
37.
APOBEC3G interferes with reverse transcription of HIV-1 independent of DNA deamination . tRNA3Lys typically binds to the HIV-1 primer binding site to initiate reverse transcription.
38.
The other major high throughput technique employed in the quantification of specific RNA molecules is quantitative PCR, after reverse transcription of the RNA to cDNA.
39.
APOBEC3G interferes with reverse transcription of HIV-1 independent of DNA deamination . tRNA3Lys typically binds to the HIV-1 primer binding site to initiate reverse transcription.
40.
The ligation step adds DNA adaptors to both ends of the small RNAs, which act as primer binding sites during reverse transcription and PCR amplification.
