The culture supernatant can yield 1 to 60 �g / ml of monoclonal antibody, which is maintained at-20 �C or lower until required.
32.
Non-compacted particles remain mostly in the liquid called " supernatant " and can be removed from the vessel thereby separating the supernatant from the pellet.
33.
Non-compacted particles remain mostly in the liquid called " supernatant " and can be removed from the vessel thereby separating the supernatant from the pellet.
34.
Proteins in the supernatant or on the outside of the cell membrane can be bound by the antibodies; this allows for living cells to be stained.
35.
Treatment of Schwann cells with the collected supernatant shows that it is a mitogenic factor and thus plays an important role in the proliferation of Schwann cells.
36.
Optimal IC and EC space perfusion rates must be achieved in order to efficiently deliver media nutrients and growth supplements, respectively, and to collect supernatant.
37.
When placed in the aqueous environment at pH 6, positively charged hemoglobin binds to the nanosheets, whereas negative albumin can be detected in the supernatant.
38.
At 100, 000 g, achieved by faster centrifuge rotation, ER sediments out of solution as a pellet but the soluble enzymes remain in the supernatant.
39.
The supernatant contains a mitogenic factor, a mitosis promoting factor, that is characterized heat and trypsin sensitivity, both of which characterize it as a peptide.
40.
This mechanism of capturing the secreting cytokine makes the ELISPOT method far more sensitive than assays that measure cytokine released into culture supernatants, for the above stated reasons.
