After noticing an unusual alignment of immune cells along the dural sinus using the meningeal whole-mount technique, the Kipnis lab found a network of vessels in the dura that expressed lymphatic endothelial cell marker proteins, including PROX1, LYVE1, and PDPN . In follow-up experiments, the vessels were also shown to extend along the length of both the superior sagittal and transverse sinuses, and ( after the performance of anatomical tracing experiments with Evans blue dye ) to directly connect to the deep cervical lymph nodes.
