| 41. | However, these different enzymes share the need to stabilize an enolate anion intermediate derived from an acyl-CoA substrate.
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| 42. | There have been conflicting results on the ionization state of the intermediate, whether it exists as the enolate or enol.
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| 43. | One topic covered in depth here, that may not belong here so completely, is the coverage of enolate formation.
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| 44. | It was proposed in 1955 that the face of the enolate, thus leading to the less stable of two diastereomers.
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| 45. | Indeed, in some cases, anionic assistance is intentionally forgone to accommodate for the production of overly sensitive enolate product.
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| 46. | Structures 2a to 2c are three resonance structures that can be drawn for this species, two of which have enolate ions.
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| 47. | In this case, the enolate is generated by direct deprotonation of the substrate with lithium diisopropyl amide ( LDA ) base.
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| 48. | The enol or enolate is itself generated from a carbonyl compound, often an aldehyde or ketone, using acid or base.
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| 49. | In any case, the second step is well described in all cases as the reaction of an enolate with an electrophile.
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| 50. | The two steps may be carried out as distinct experimental operations if the initially formed enolate is protected after ?-addition.
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