An example of the ion-exchange chromatography is given by the NTRC using sulfonated polystyrene as a matrix, adding the amino acids in acid solution and passing a buffer of steadily increasing pH through the column.
42.
During World War II, Cohn worked on the Manhattan Project in Oak Ridge and helped create plutonium for the bomb dropped on Hiroshima by using a process known as ion exchange chromatography in the splitting of uranium atoms.
43.
However, if a direct " catcher " gold foil is used, the process is complicated by the need to separate out the gold using anion-exchange chromatography before isolating the nobelium by elution from chromatographic extraction columns using HDEHP.
44.
However, if the foil is made of gold and thin enough, it is enough to simply dissolve the gold in aqua regia before separating the trivalent actinides from the gold using anion-exchange chromatography, the eluant being 6 M hydrochloric acid.
45.
It has historically been very difficult and expensive to separate rare earths from each other in their ores but ion-exchange chromatography methods developed in the late 20th century have greatly brought down the cost of production of all rare-earth metals and their chemical compounds.
46.
Ion-exchange chromatography utilizes a resin such as chelex 100 in which iminodiacetate residues, attached to a polymer backbone, form chelate complexes of differing strengths with different metal ions, allowing the ions such as Cu 2 + and Ni 2 + to be separated chromatographically.
47.
Despite this selectivity, excess amounts of an ion with a lower selectivity introduced to the column would cause the lesser ion to bind more to the stationary phase as the selectivity coefficient allows fluctuations in the binding reaction that takes place during ion exchange chromatography.
48.
Another clinical application of ion chromatography is in the rapid anion exchange chromatography technique used to separate creatine kinase ( CK ) isoenzymes from human serum and tissue sourced in autopsy material ( mostly CK rich tissues were used such as cardiac muscle and brain ).
49.
Either cation exchange chromatography is used at a low enough pH that the desired antibody binds to the column while anions flow through, or anion exchange chromatography is used at a high enough pH that the desired antibody flows through the column while anions bind to it.
50.
Either cation exchange chromatography is used at a low enough pH that the desired antibody binds to the column while anions flow through, or anion exchange chromatography is used at a high enough pH that the desired antibody flows through the column while anions bind to it.
