An example is the secretion of hemolysin ( HlyA ) from " E . coli " where the inner membrane ABC transporter HlyB interacts with an inner membrane fusion protein HlyD and an outer membrane facilitator TolC . TolC allows hemolysin to be transported across the two membranes, bypassing the periplasm.
42.
An example is the secretion of hemolysin ( HlyA ) from " E . coli " where the inner membrane ABC transporter HlyB interacts with an inner membrane fusion protein HlyD and an outer membrane facilitator TolC . TolC allows hemolysin to be transported across the two membranes, bypassing the periplasm.
43.
Hemolysins have proved to be a damaging factor for vital organs, through the activity of " Staphylococcus aureus " . " S . aureus " is a dangerous pathogen that may lead cells to necrotizing infections usually recognized by a massive inflammatory response leading to tissue damage or even tissue destruction.
44.
Of negative biosafety aspects tested, it was shown that there was no production of enterotoxins ( Shiga toxins, heat-stable and heat-labile toxins ), no production of cytotoxins ( CNF ), no invasiveness, no pathogenic adhesion factors ( e . g . no CFA I / II, P, M and S fimbriae ), no hemolysins, no serum resistance ( i . e . no risk of sepsis ), no uropathogenicity and no antibiotic-resistance genes.
45.
In most exporters, the N-terminal transmembrane domain and the C-terminal ABC domains are fused as a single polypeptide chain, arranged as TMD-NBD-TMD-NBD . An example is the " E . coli " hemolysin exporter HlyB . Importers have an inverted organization, that is, NBD-TMD-NBD-TMD, where the ABC domain is N-terminal whereas the TMD is C-terminal, such as in the " E . coli"
