Further optimization can be achieved by designing oligos that target the lagging strand in DNA replication because targeting the lagging strand results in a higher frequency of recombinants.
42.
Further optimization can be achieved by designing oligos that target the lagging strand in DNA replication because targeting the lagging strand results in a higher frequency of recombinants.
43.
High processivity is in part ensured by ring-shaped proteins referred to as'clamps'that help replicative polymerases stay associated with the leading and lagging strands.
44.
The discontinuous stretches of DNA replication products on the lagging strand are known as Okazaki fragments and are about 100 to 200 bases in length at eukaryotic replication forks.
45.
In DNA replication, RNA primers must be inserted along the lagging strand so that DNA polymerase is able to synthesize the strand in the 5 to 3 direction.
46.
The leading strand is continuously extended from the primer by a DNA polymerase with high processivity, while the lagging strand is extended discontinuously from each primer forming Okazaki fragments.
47.
The " oriC " site in " E . coli " has three primer for the leading strand and to add RNA primers on the lagging strand.
48.
This means that, while polymerisation of the leading strand proceeds, polymerisation of the lagging strand only occurs after enough of the lagging strand has been unwound by the helicase.
49.
This means that, while polymerisation of the leading strand proceeds, polymerisation of the lagging strand only occurs after enough of the lagging strand has been unwound by the helicase.
50.
The single stranded ( ss ) DNA synthesized during lagging strand synthesis can be targeted by ss-DNA damaging agents as well as is a selective target for APOBEC mutations.
