Once an oligonucleotide of appropriate length has been synthesized from the elongation step of primer synthesis, DnaG transfers the newly synthesized primer to DNA polymerase III for it to synthesize the DNA leading strand or Okazaki fragments for the lagging strand.
42.
On the leading strand DNA replication proceeds continuously along the DNA molecule as the parent double-stranded DNA is unwound, but on the lagging strand the new DNA is made in installments, which are later joined together by a DNA ligase enzyme.
43.
The primosome consists of seven proteins : DnaG primase, DnaB helicase, DnaC helicase assistant, DnaT, PriA, Pri B, and PriC . At each replication fork, the primosome is utilized once on the leading strand of DNA and repeatedly, initiating each Okazaki fragment, on the lagging DNA strand.
44.
Therefore, positive GC skew represents richness of G over C and the negative GC skew represents richness of C over G . As a result one expects to see a positive GC skew and negative AT skew in the leading strand, and a negative GC skew and a positive AT skew in the lagging strand.
45.
Evangelicalism has therefore been described as " the third of the leading strands in American Protestantism, straddl [ ing ] the divide between fundamentalists and liberals . " While the North American perception is important to understand the usage of the term, it by no means dominates a wider global view, where the fundamentalist debate was not so influential.
46.
However, in most prokaryotes ( e . g . " E . coli " ) and some archaea ( e . g . " Sulfolobus solfataricus " ), nucleotide compositions are asymmetric between the leading strand and the lagging strand : the leading strand contains more Guanine ( G ) and Thymine ( T ), whereas the lagging strand contains more Adenine ( A ) and Cytosine ( C ).
47.
However, in most prokaryotes ( e . g . " E . coli " ) and some archaea ( e . g . " Sulfolobus solfataricus " ), nucleotide compositions are asymmetric between the leading strand and the lagging strand : the leading strand contains more Guanine ( G ) and Thymine ( T ), whereas the lagging strand contains more Adenine ( A ) and Cytosine ( C ).
48.
The dimerisation of the replicative polymerases solves the problems related to efficient synchronisation of leading and lagging strand synthesis at the replication fork, but the tight spatial-structural coupling of the replicative polymerases, while solving the difficult issue of synchronisation, creates another challenge : dimerisation of the replicative polymerases at the replication fork means that nucleotide synthesis for both strands must take place at the same spatial location, despite the fact that the lagging strand must be synthesised backwards relative to the leading strand.
