The scientists seeking permission to alter the virus genetically want to introduce a marker gene that would glow green under a fluorescent light to indicate whether a virus was still alive after being treated by an experimental antiviral drug.
42.
When mammalian cells ( e . g . human or mouse cells ) are used, a similar strategy is used, except that the marker gene ( in this case typically encoded as part of the kanMX cassette ) confers resistance to the antibiotic Geneticin.
43.
Brinster and his colleagues found that they could gather stem cells from the testes of mice that had marker genes in all their cells, micro-inject the sperm stem cells into the testes of other animals and see the marker genes'effects in the next generation.
44.
Brinster and his colleagues found that they could gather stem cells from the testes of mice that had marker genes in all their cells, micro-inject the sperm stem cells into the testes of other animals and see the marker genes'effects in the next generation.
45.
But Lavanchy said the experiments that the advisory committee rejected last year were " fundamentally different " than those recommended last week and that the insertion of the marker gene in the experiments now being proposed would not alter the ability of variola virus to cause disease.
46.
The 626-member European Parliament is expected to approve proposals that include stricter labeling and monitoring of genetically altered foods, feeds, and pharmaceutical products; phasing out the use of antibiotic marker genes in altering food plants, and setting up a public registry where consumers can trace genetically modified foods.
47.
PlasMapper accepts plasmid / vector DNA sequence as input ( FASTA format ) and uses sequence pattern matching and BLAST sequence alignment to automatically identify and label common promoters, terminators, cloning sites, restriction sites, reporter genes, affinity tags, selectable marker genes, origins of replication and open reading frames.
48.
In gene therapy, gene delivery vectors, such as viruses, can be imaged according either to their particle biodistribution or their PET and latter means visualising the marker gene of gene delivery vector to be visible by the means of immunohistochemical methods, optical imaging or even by PCR . Non-invasive imaging has gained popularity as the imaging equipment has become available for research use from clinics.
