Several different protein engineering techniques have been employed to improve both the activity and specificity of the nuclease domain used in ZFNs.
42.
The RNase III Drosha is the core nuclease that executes the initiation step of microRNA ( miRNA ) processing in the nucleus.
43.
In addition, boranophosphates have increased nuclease resistance without affecting activation of RNase H cleavage of RNA in RNA : boranophosphate hybrids.
44.
The nuclease domain is responsible for physical cleavage of DNA strands and may introduce either single stranded or double-stranded breaks.
45.
Additional developments with zinc finger nuclease technology in 2009 led to the first knockout rat with targeted, germline-transmitted mutations.
46.
In 2004, Qiu et al . developed a mutation detection technology based on CEL II, also known as Surveyor Nuclease.
47.
Surveyor nuclease also has a 52 exonuclease activity that attacks the ends of double stranded DNA increasing background signal during extended incubation.
48.
MCM8 / MCM9 interacts with MRN and is required for the nuclease action and stable association of MRN with double-strand breaks.
49.
A nuclease that functions by removing nucleotides from the ends of the DNA molecule is called an "'exonuclease . "'
50.
MMEJ starts with short-range end resection by MRE11 nuclease on either side of a double-strand break to reveal microhomology regions.
