Such a process is relatively fast and the resulting double-stranded polynucleotide is much more stable than the single single-stranded one since each monomer is bound not only along the sugar phosphate backbone, but also through inter-strand bonding between the bases.
42.
This means that the conformational stability of a highly ordered polynucleotide ( DNA, RNA etc . ) can be manipulated either by the addition of a cyclobuxine-like molecules in small increments, or in its presence at quite low concentration, by fluctuations in ionic strength.
43.
The second linker interacts with polynucleotide kinase phosphatase ( PNKP ) ( that processes DNA broken ends during base excision repair ), aprataxin ( active in single-strand DNA repair and non-homologous end joining ) and a third protein designated aprataxin-and PNKP-like factor.
44.
Crick, however, knowing the Fourier transforms of Bessel functions that represent the X-ray diffraction patterns of helical structures of atoms, correctly interpreted further one of Franklin's experimental findings as indicating that DNA was most likely to be a double helix with the two polynucleotide chains running in opposite directions.
45.
:Kinases are enzymes that facilitate the addition of phosphate groups to other molecules; a'kinased'oligonucleotide is therefore one to which a phosphate has been added ( generally to the 5'end ) through the use of an enzyme ( most often T4 or T7 polynucleotide kinase ) . talk ) 15 : 49, 30 April 2012 ( UTC)
46.
They hypothesized that if discontinuous replication, involving short DNA chains linked together by polynucleotide ligase, is the mechanism used in DNA synthesis, then " newly synthesized short DNA chains would accumulate in the cell under conditions where the function of ligase is temporarily impaired . " " E . coli " were infected with bacteriophage T4 that produce temperature-sensitive polynucleotide ligase.
47.
They hypothesized that if discontinuous replication, involving short DNA chains linked together by polynucleotide ligase, is the mechanism used in DNA synthesis, then " newly synthesized short DNA chains would accumulate in the cell under conditions where the function of ligase is temporarily impaired . " " E . coli " were infected with bacteriophage T4 that produce temperature-sensitive polynucleotide ligase.
48.
Here, " C l " is the total concentration of all polynucleotide chains belonging to a hypercycle " H l ", " C " is the total concentration of polynucleotide chains belonging to all hypercycles, " q l " is the rate of growth, and " & phi; " is a dilution flux that guarantees that the total concentration is constant.
49.
Here, " C l " is the total concentration of all polynucleotide chains belonging to a hypercycle " H l ", " C " is the total concentration of polynucleotide chains belonging to all hypercycles, " q l " is the rate of growth, and " & phi; " is a dilution flux that guarantees that the total concentration is constant.
50.
As shown in figure 3, in NHEJ, the " Ku70 / Ku80 " heterodimer and DNA-dependent protein kinase " ( DNA-PK ) ", for bringing DNA fragments end together, the Artemis nuclease and polynucleotide kinase 3'phosphatase " ( PNKP ) ", for the end processing, X family DNA polymerases " ( Pol ? and Pol ? ) " and terminal deoxynucleotidyl transferase " ( TdT ) ", for DNA synthesis, and " XLF / XRCC4 / LigIV " complex, for completing the repair and joining the ends via a phosphodiester bond, are the protein complexes involved in DSB repair process in many higher organisms.
