FAN1 recruitment and its consequent activity restrain DNA replication fork progression and prevent chromosome abnormalities from occurring when DNA replication forks stall.
42.
Replication of the DNA separating the opposing replication forks, leaves the completed chromosomes joined as catenanes or topologically interlinked circles.
43.
They also found chromosomal rearrangements : DNA breaks may origin by replication fork collapse or hydrolysis of RN on opposing DNA strands.
44.
Three of them form the FA AP24-MHF1 / 2 complex which recognizes the ICL ( from stalled replication forks ).
45.
After DnaB translocates to the apex of each replication fork, the helicase both unwinds the parental DNA and interacts momentarily with primase.
46.
As replication occurs, DNA ahead of the replication bubble becomes positively supercoiled, while DNA behind the replication fork becomes entangled forming precatenanes.
47.
Pause sites can be identified, merged replication forks resolved, and the frequency of origin firings in different time periods to be studied.
48.
Eukaryotic origins of replication control the formation of a number of protein complexes that lead to the assembly of two bidirectional DNA replication forks.
49.
To thwart these problems, the eukaryotic replisome contains specialized proteins that are designed to regulate the helicase activity ahead of the replication fork.
50.
Checkpoint proteins are also involved in some DNA repair pathways, while they stabilize the structure of the replication fork to prevent further damage.
