| 1. | Restriction Endonuclease enzymes play a very important role in modern molecular cloning techniques.
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| 2. | A restriction endonuclease functions by " scanning " the length of a DNA molecule.
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| 3. | An example of a Type I restriction endonuclease.
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| 4. | However, if genomic DNA is treated with a particular restriction endonuclease many DSBs will be created.
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| 5. | Most commonly used restriction enzymes are Type II restriction endonuclease ( See article on Restriction enzymes for examples ).
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| 6. | The plasmid contains a genetic sequence corresponding to the recognition site of a restriction endonuclease, such as EcoR1.
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| 7. | Sickle-cell gene mutation probably arose spontaneously in different geographic areas, as suggested by restriction endonuclease analysis.
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| 8. | When examining the preferential double strand cleavage of DNA, the restriction endonuclease PstI bind to pSM1 plasmid DNA.
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| 9. | The sequence altered by the mutation eliminates the recognition site for the restriction endonuclease MstII that recognizes the nucleotide sequence.
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| 10. | Ronald W . Davis first discovered sticky ends as the product of the action of EcoRI, the restriction endonuclease.
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