cloning vector वाक्य
उदाहरण वाक्य
मोबाइल
- The gene, once cloned into the cloning vector ( called entry clone in this method ), may be conveniently introduced into a variety of expression vectors by recombination.
- Once cut, scientist can then insert the desired DNA fragment possessing " sticky ends " into a circular DNA molecule, and ligate them together to create an engineered cloning vector.
- Some plasmids contain an M13 bacteriophage origin of replication and may be used to generate single-stranded DNA . These are called phagemid, and examples are the pBluescript series of cloning vectors.
- The number of clones that constitute a genomic library depends on ( 1 ) the size of the genome in question and ( 2 ) the insert size tolerated by the particular cloning vector system.
- Restriction mapping is a very useful technique when used for determining the orientation of an insert in a cloning vector, by mapping the position of an off-center restriction site in the insert.
- For example, pBR322 is a low copy number plasmid ( ~ 20 copies / cell ) from which several very high copy number cloning vectors ( ~ 1000 copies / cell ) have been derived.
- TetA is also present in the widely used E . coli cloning vector pBR322 ( where the sequence that confers resistance, containing only the TetA gene, is also often referred to as Tet R ).
- Cloning is generally first performed using " Escherichia coli ", and cloning vectors in " E . coli " include plasmids, bacteriophages ( such as phage ? ), cosmids, and bacterial artificial chromosomes ( BACs ).
- For example, if a DNA sample is going to be used in a cloning experiment, the step that follows its running on an agarose gel is to ligate ( covalently link ) to a cloning vector ( most likely a plasmid ).
- Example of cloning vectors used for this test are pUC19, pBluescript, pGem-T Vectors, and it also requires the use of specific " E . coli " host strains such as DH5? which carries the mutant " lacZ?M15 " gene.
- Cloning vectors without promoter and RBS for the cloned DNA sequence are sometimes used, for example when cloning genes whose products are toxic to " genomic or cDNA library of clones since the cloned genes are normally subcloned into a more appropriate expression vector if their expression is required.
- This may be useful in TA cloning, whereby a cloning vector ( such as a plasmid ) that has a T ( thymine ) 3'overhang is used, which complements with the A overhang of the PCR product, thus enabling ligation of the PCR product into the plasmid vector.
- These unique features and other features gave significance of the T-even phages, this includes transduction which is responsible for transfer of drug resistant features, lysogenic conversion is responsible for acquisition of new characteristics such as the formation of new enzymes, random insertion into bacterial chromosome can induce insertional mutation, epidemiological typing of bacteria ( phage typing ), phages are used extensively in genetic engineering where they serve as cloning vectors.
- अधिक वाक्य: 1 2
cloning vector sentences in Hindi. What are the example sentences for cloning vector? cloning vector English meaning, translation, pronunciation, synonyms and example sentences are provided by Hindlish.com.