reverse transcription वाक्य
उदाहरण वाक्य
मोबाइल
- When a retrovirus enters a host cell's cytoplasm, it undergoes processes such as capsid uncoating and reverse transcription.
- Generally this is achieved through the use of reverse transcription of the RNA followed by polymerase chain reaction.
- This step does not have to be necessarily carried out before the ligation and reverse transcription steps outlined below.
- A direct confirmation can be obtained by reverse transcription polymerase chain reaction where the genome of the virus is amplified.
- It is predicted that reverse transcription is also negatively affected by APOBEC3G binding to viral RNA and causing steric alterations.
- Hepatitis B is one of a few known non-retroviral viruses which use reverse transcription as a part of its replication process.
- Within the cytoplasm the viral reverse transcriptase enzyme performs reverse transcription of the viral RNA genome to create a viral DNA genome.
- The first step in RACE is to use reverse transcription to produce a cDNA copy of a region of the RNA transcript.
- The product line includes various subbrands of biotechnology products, such as machines and consumables for polymerase chain reaction, reverse transcription, cloning, immunoassays.
- A cDNA library is constituted by the cDNA produced through reverse transcription of mRNA collected from specific cells of types of cell.
- CDNAs obtained from reverse transcription of mRNA population extracted from the cells in varying physiological and experimental conditions are used as immobilized probes.
- The revised hypothesis was later formulated, in part, to accommodate reverse transcription ( which both Howard Temin and David Baltimore discovered in 1970 ).
- Using Reverse transcription polymerase chain reaction and a more rigorous nested primer system they found evidence for splice variants of the ETV6-NTRK3 fusion gene.
- They also concluded from quantitative reverse transcription-PCR ( qRT-PCR ) analysis that the five consecutive genes are co-regulated by the same BCG1-calcineurim transduction pathway.
- CaMV contains a circular double-stranded DNA molecule of about 8.0 kilobases, interrupted by nicks that result from the actions of RNAse H during reverse transcription.
- This is particularly important in retroviral infections since viral integration into the host genome occurs quickly after entry and reverse transcription and is largely irreversible.
- APOBEC3G interferes with reverse transcription of HIV-1 independent of DNA deamination . tRNA3Lys typically binds to the HIV-1 primer binding site to initiate reverse transcription.
- The other major high throughput technique employed in the quantification of specific RNA molecules is quantitative PCR, after reverse transcription of the RNA to cDNA.
- APOBEC3G interferes with reverse transcription of HIV-1 independent of DNA deamination . tRNA3Lys typically binds to the HIV-1 primer binding site to initiate reverse transcription.
- The ligation step adds DNA adaptors to both ends of the small RNAs, which act as primer binding sites during reverse transcription and PCR amplification.
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