| 1. | Acridine orange is useful for enumerating the microbes in a sample.
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| 2. | Like the related molecule pyridine and quinoline, acridine is mildly basic.
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| 3. | It is a precursor to certain acridine dyes as well.
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| 4. | There are no commercial applications of acridines but at one time acridine dyes were popular.
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| 5. | Examples include ethidium bromide and acridine.
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| 6. | Acridine orange direct count ( AODC ) methodology has been used in the enumeration of landfill bacteria.
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| 7. | Condensation of 2, 4-diaminotoluene with acetaldehyde gives the acridine dye called Basic Yellow 9.
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| 8. | Acridine orange binding with the nucleic acid occurs in both living and dead bacteria, also other microorganisms.
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| 9. | The use of polyphosphoric acid will give acridine products at a lower temperature, but also with decreased yields.
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| 10. | Acridine orange can be used in conjunction with ethidium bromide to differentiate between viable, apoptotic and necrotic cells.
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