He produced an artificial autotetraploid grass from the diploid species " Ehrharta erecta " through treatment with the chromosome doubling agent colchicine.
2.
"Pseudoroegneria spicata " is most commonly found as a diploid ( 2n = 14 ), although autotetraploid forms ( 4n = 28 ) have been found in eastern Washington and northern Idaho.
3.
The relationship between the two species has never been closely analyzed, but it does not appear that " P . grandiflorus " is simply an autotetraploid of " P . tenuifolius ".
4.
It has two subspecies : one diploid, which is actually the original fern, " "'Asplenium petrarchae ssp . bivalens " "'with a chromosome number of 2n = 72 and one autotetraploid by spontaneous doubling of their genome, " "'Asplenium petrarchae ssp . petrarchae " "', very more abundant than the subspecies " bivalens ".