Hence the resazurin / diaphorase / NADPH system can be used to detect NADH, NADPH, or diaphorase level, and any biochemical or enzyme activity that is involved in a biochemical reaction generating NADH or NADPH.
2.
Hence the resazurin / diaphorase / NADPH system can be used to detect NADH, NADPH, or diaphorase level, and any biochemical or enzyme activity that is involved in a biochemical reaction generating NADH or NADPH.
3.
Diaphorase It normally contributes only a small percentage of the red blood cell's reducing capacity, but can be pharmacologically activated by exogenous cofactors ( such as methylene blue ) to 5 times its normal level of activity.
4.
Methemoglobinemia can also arise in patients with pyruvate kinase deficiency due to impaired production of NADH the essential cofactor for diaphorase I . Similarly, patients with glucose-6-phosphate dehydrogenase deficiency may have impaired production of another co-factor, NADPH.
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