| 1. | Dithizone may be prepared by reacting phenylhydrazine with carbon disulfide, followed by reaction with potassium hydroxide.
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| 2. | Exocrine tissue also present in the preparations does not bind dithizone, and is therefore not stained.
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| 3. | Dithizone binds zinc ions present in the islet's beta cells, and therefore stains the islets red.
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| 4. | Dithizone is used to assess the purity of human pancreatic islet preparations used for transplantation into patients with type 1 diabetes.
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