Traditionally , somatic nuclear transfer in mammal is completed by micromanipulation . but micromanipulation is evidently damaging to cytoplast cytoskelton , causing the lost of some important factors involved in programming of donor karyoplast , reducing the volume of cytoplasm
2.
Since the success of dolly , the first cloned sheep with the adult somatic cells as karyoplast donor , new approaches have been developed for nuclear transfer technology . here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture . enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
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