Pectinesterase catalyses the de-esterification of pectin into pectate and methanol.
2.
Pectinesterase is inactivated by heating the juice for 1 minute at 90 �C.
3.
Plant pectinesterases are regulated by pectinesterase inhibitors, which are ineffective against microbial enzymes.
4.
In plants, pectinesterase plays an important role in cell wall metabolism during fruit ripening.
5.
Recent studies have shown that the manipulation of pectinesterase expression can influence numerous physiological processes.
6.
Pectinesterase has also been shown to play a role in a plants response to pathogen attack.
7.
Pectinesterase action on the components of the plant cell wall can produce two diametrically opposite effects.
8.
Thus, in order to keep the orange juice cloud intact, it is vital to inactivate pectinesterase.
9.
Pectinesterase functions primarily by altering the localised pH of the cell wall resulting in alterations in cell wall integrity.
10.
Several pectinesterase isoforms differing in molecular weight, isoelectric point and biochemical activity have been identified in dicotyledonous plants.
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