Fertilization occurs once the conidium of a different mating type makes contact with the trichogyne, a structure of the protoperithecium.
2.
The two cells from both the protoperithecium and conidium fuse, yet their nuclei remain separate until ascus formation has occurred.
3.
The protoperithecium consists of an ascogonium, a coiled multicellular hypha that is enclosed in a knot-like aggregation of hyphae.
4.
Protoperithecia formation occurs in environments of low nitrogen The protoperithecium will act as the female gamete as the recipient of a nucleus while the conidium which will act as the male gamete donating a nucleus.
5.
Once a haploid fungus within N . crassa has attracted or has been attracted to a mate of a different mating type, it will develop either a protoperithecium or conidium to sexually fertilize or both structures to self-fertilize.
6.
However, as a recipient, the protoperithecium of both the " A " and " a " strains can be thought of as the female structure, and the fertilizing conidium can be thought of as the male participant.
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