Multiwell plates are used initially to grow the hybridomas, and after selection, are changed to larger tissue culture flasks.
12.
Initially, murine antibodies were obtained by hybridoma technology, for which Jerne, K�hler and Milstein received a Nobel prize.
13.
A monoclonal antibody against the myc epitope, named 9E10, is available from the non-commercial Developmental Studies Hybridoma Bank.
14.
The next stage is a rapid primary screening process, which identifies and selects only those hybridomas that produce antibodies of appropriate specificity.
15.
Hybridomas are cells obtained by fusing tumor cells ( which can divide infinitely ) and immunoglobulin-producing lymphocytes ( plasma cells ).
16.
The organization holds a collection of more than 3, 000 human and animal cell lines and an additional 1, 200 hybridomas.
17.
"' Hybridoma technology "'is a method for producing large numbers of identical antibodies ( also called monoclonal antibodies ).
18.
Cell extracts from other sources such as hyperthermophiles, hybridomas, Xenopus oocytes, insect, mammalian and human cells have also been used.
19.
Prize committees then began to include Kohler in the kudos they showered down on the monoclonal antibody technique, also known as the hybridoma technique.
20.
(v ) production of monoclonal antibodies by producing hybridoma ( hybrid cells between an immortalised cell and an antibody producing lymphocyte ), etc.