| 1. | This medium is selective for fused ( hybridoma ) cells.
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| 2. | The hybridomas can be grown indefinitely in a suitable cell culture medium.
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| 3. | The plasma cells in the culture eventually die from senesence, leaving pure hybridoma cells.
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| 4. | Hybridoma technology has been replaced by recombinant DNA technology, transgenic mice and phage display.
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| 5. | The medium must be enriched during " in vitro " selection to further favour hybridoma growth.
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| 6. | These CD4 + CD25 + hybridomas resemble in function the naturally occurring CD4 + CD25 + regulatory cells.
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| 7. | Hybridomas can be expanded to produce large quantities of immunoglobulins with a given unique specificity ( monoclonal antibodies ).
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| 8. | One problem is to single out the hybridomas from the large excess of unfused cells after the cell fusion.
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| 9. | This maintains the well-being of the hybridomas and provides enough cells for cryopreservation and supernatant for subsequent investigations.
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| 10. | IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone.
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