Acridine orange is a versatile fluorescence dye used to stain acidic vacuoles ( lysosomes, endosomes, and autophagosomes ), RNA, and DNA in living cells.
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Apoptosis was evaluated by flow cytometry on PBMC from HIV infected individuals ( cells were stained with acridine orange and ethidium bromide ) ( n = 6 ).
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When an acridine orange binds with DNA, it exhibits an excitation maximum at 502 nm ( cyan ) and an emission maximum at 525 nm ( green ).
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As a result, DNA intercalators are often carcinogen s, with benzopyrene | benzopyrene diol epoxide, acridine s, aflatoxin and ethidium bromide being well-known examples.
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You'll find a lot of useful information using Google ( or other search engine, even Pubmed ) with the example of Acridine orange and " vital ".
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Later on, Lauer, Reller and Mirret performed a similar study, compared acridine orange with the Gram stain for detecting the microorganisms in cerebrospinal fluid and other clinical materials.
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Additionally, acridine orange may be used on blood samples to cause bacterial DNA to fluoresce, aiding in clinical diagnosis of bacterial infection once serum and debris have been filtered.
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"' Acridine "'is an organic compound and a nitrogen heterocycle with the formula C 13 H 9 N . Acridines are substituted derivatives of the parent ring.
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The results showed that the acridine orange is a simple, inexpensive, rapid staining procedure that appeared to be more sensitive than the Gram stain for detecting microorganism in clinical materials.
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Acridine Yellow can be used for this purpose, together with 9, 10-bis ( phenylethynyl ) anthracene, and a scale of different azo dyes, aromatic hydrocarbons, and other dyes.
