Negative supercoiling is also thought to favour the transition between B-DNA and Z-DNA, and moderate the interactions of DNA binding proteins involved in gene regulation.
22.
DNA gyrase was incubated with the MurI enzyme and then added to a sample of DNA; the results of this experiment showed inhibition of supercoiling activity when MurI was present.
23.
In supercoiling assays, coiled DNA is separated in the first dimension and denatured by a DNA intercalator ( such as ethidium bromide or the less carcinogenic chloroquine ) in the second.
24.
"' DNA supercoiling "'refers to the over-or under-winding of a DNA strand, and is an expression of the strain on that strand.
25.
Supercoiling plays an important part in polymerase activity because of the unwinding and rewinding of DNA . Because regions of DNA in front of RNAP are unwound, there are compensatory positive supercoils.
26.
Topoisomerases introduce both single-and double-strand breaks in the course of changing the DNA's state of supercoiling, which is especially common in regions near an open replication fork.
27.
Supercoiling is important in a number of biological processes, such as compacting DNA, and by regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism and possibly gene expression.
28.
Supercoiling is important in a number of biological processes, such as compacting DNA, and by regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism and possibly gene expression.
29.
Octameric protein complexes called nucleosomes are responsible for the amount of supercoiling of DNA, and these complexes can be temporarily modified by processes such as phosphorylation or more permanently modified by processes such as methylation.
30.
"' Jerome Vinograd "'( February 9, 1913 July 7, 1976 ) was an American biochemist who developed density gradient ultracentrifugation and contributed to the understanding of DNA supercoiling.
