avidin वाक्य
उदाहरण वाक्य
मोबाइल
- :I'm assuming there are strept ? avidin beads like this being bound to biotinylated protein with varying numbers of moieties attached.
- For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.
- ProdiGene struck agreements with Sigma to distribute ProdiGene's corn-produced aprotinin, trypsin, beta-glucuronidase ( GUS ), and avidin.
- This allows elution from the avidin matrix to occur under milder, non-denaturing conditions, using low concentrations of biotin or low pH conditions.
- For a single high affinity biotin binding site without crosslinking, a monovalent version of avidin's distant relative, streptavidin, may be used.
- For example, avidin-displaying baculoviruses could be imaged in rat brain by coating them with biotinylated iron particles, rendering them visible in MR imaging.
- Avidin's affinity for biotin is exploited in wide-ranging biochemical assays, including western blot, ELISA, ELISPOT and pull-down assays.
- The non-reversible nature of the avidin-biotin complex can limit avidin s application in affinity chromatography applications where release of the captured ligand is desirable.
- Like avidin itself, NeutrAvidin is a tetramer with a strong affinity for biotin ( " K " d = 10 " 15 M ).
- Due to the high affinity of biotin to avidin and streptavidin, the radiotherapy accumulates where the antibody is, namely in the tumour, where it delivers its radioactivity.
- The biotin-binding properties of avidin were exploited during the development of idrabiotaparinux, a long-acting low molecular weight heparin used in the treatment of venous thrombosis.
- This antibody has also been treated with biotin, a Vitamin-D derivative that acts like a kind of molecular Velcro when exposed to avidin, a protein derived from eggs.
- Further amplification can be achieved if the secondary antibody is conjugated to several biotin molecules, which can recruit complexes of avidin-, streptavidin-or NeutrAvidin protein-bound enzyme.
- While working to characterize the yeast growth factor that would become known as biotin, Snell and coworkers discovered the egg white protein avidin, which binds biotin with extremely high affinity.
- By adding a biotin moiety to the idraparinux molecule, idrabiotaparinux was formed; its anticoagulant activity in the setting of a bleeding event can be reversed through an intravenous infusion of avidin.
- While there are some non-covalent interactions that may survive 2D-PAGE ( biotin-( strept ) avidin, for example ) I wouldn't rely on this happening.
- The thermal stability and biotin binding activity of avidin are of both practical and theoretical interest to researchers, as avidin's stability is unusually high and avidin is an antinutrient in human food.
- The thermal stability and biotin binding activity of avidin are of both practical and theoretical interest to researchers, as avidin's stability is unusually high and avidin is an antinutrient in human food.
- The thermal stability and biotin binding activity of avidin are of both practical and theoretical interest to researchers, as avidin's stability is unusually high and avidin is an antinutrient in human food.
- The natural function of avidin in eggs is not known, although it has been postulated to be made in the oviduct as a bacterial growth-inhibitor, by binding biotin helpful for bacterial growth.
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