Trypsinization is often used to passage cells to a new vessel.
2.
When trypsinization process is complete the cells will be in suspension and appear rounded.
3.
"' Trypsinization "'is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured.
4.
Trypsinization is often done to permit passage of the cells to a new container, observation for experimentation, or reduction of the degree of confluency in the flask by removal of a percentage of the cells.
5.
In 2D, the cells must undergo regular trypsinization in order to provide them with sufficient nutrients for normal cell growth . 3D spheroids have been cultured in a lab setting for up to 302 days while still maintaining healthy, non-cancerous growth.
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